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In an aspect of second embodiment, the said insoluble tag is optionally linked to a nucleotide sequence encoding affinity tag to give Formula (III), Affinity Tag Insoluble tag Fusion tag Semapeptide (Nucleotide Sequence) Formula (III) In another aspect of second embodiment, present invention involves the preparation of fusion tag construct of Formula (III) comprising fusion of amino terminal of synthetic nucleotide of semapeptide with carboxyl terminal of fusion tag construct