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This observation was further confirmed in yet another study by the same group, where KEKS food [standard chow with mixed in ketone esters and ketone salts (110)] supplementation decreased the lipopolysaccharide-induced elevation of SWD in the same rat model, while A 1 R, but not A 2A R antagonism abolished the anti-seizure effect of KEKS supplementation (111)

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RNA sequencing To analyse the role of KAT6A in gene regulation in the developing brain, E12.5 dorsal telencephalon tissue was dissected and frozen, and E16.5 cortical neurons were isolated and total RNA was extracted using an RNeasy mini kit (QIAGEN) according to the manufacturers instructions including the optional DNase I digestion step

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