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J Biol Chem 264:1020510209 Quiroz Y, Ferrebuz A, Vaziri N, Rodriguez-Iturbe B (2009) Effect of chronic antioxidant therapy with superoxide dismutase-mimetic drug, tempol, on progression of renal disease in rats with renal mass reduction

Subsequently, the peptides were separated on a Pepmap100 RSLC C18 column (2.0 m, 75 m 150 mm, Thermo Fisher Scientific) using a multistep gradient of 2% to 40% acetonitrile with 0.1% formic acid over a period of 150 min