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After seeding the cells in a 24-well plate for 24 h, they were transfected with either the control lentivirus (Lv-NC), PPARa over-expression lentivirus (Lv -PPARa , GV341, Ubi-MCS-3FLAG-SV40-puromycin), or PPARa shRNA lentivirus (Lv -PPARa RNAi, GV112, hU6-MCS-CMV-Puromycin) at a multiplicity of infection (MOI) of 50, the details of Lv -PPARa RNAi could be found in Table 1

Catalase therapy corrects oxidative stress-induced pathophysiology in incipient diabetic retinopathy

These findings not only provide deeper insight into the molecular foundations of aging but also open promising avenues for research into interventions aimed at extending healthspan and investigating age-associated diseases