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RNA isolation, transcriptome, and RT-qPCR analyses To isolate total RNA, mid-exponential-phase cultures of strain MBM, grown on DEHP or succinate as the sole carbon source, was subjected to TRIzol reagent treatment according to the manufacturers instructions (Invitrogen, USA) following the method described earlier [71] RNase-free DNase I (Thermo Scientific, USA) treatment was done for 1 h at 37 C to remove DNA contaminants present in the RNA samples, followed by inactivation of the enzyme with EDTA at 70 C for 10 min

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