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Semaglutide-induced weight loss improves mitochondrial energy efficiency in skeletal muscle

Normally, BCAAs (e.g., valine, leucine and isoleucine) can be converted to their corresponding branched-chain keto acids that in turn can be converted to branched-chain CoA, which can also bind carnitine to form branched-chain acylcarnitines (BCACs), such as propionylcarnitine (C3-carnitine), succinylcarnitine (C4DC-carnitine) and isovalerylcarnitine (isoC5-carnitine), which are involved in the energy production process of cells [10]

This mix was then centrifuged at 2500 g for 10 min to separate the nuclear membranes from the matrix