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Isolated RNA was purified using DNase I (Sigma) before conversion to cDNA using the GoScript Reverse Transcription system (Promega)

ROS have been detected in cochlear tissue immediately after noise exposure (Yamane et al., 1995) and seen to persist for 710 days after, spreading from the basal end of the organ of Corti to the apical turn

Intervention (for example, the medical product, behavior, or procedure being studied)

Mechanism [edit] Glutathione synthase catalyzes the chemical reaction ATP + gamma-L-glutamyl-L-cysteine + glycine ADP + phosphate + glutathione The 3 substrates of this enzyme are ATP, gamma-L-glutamyl-L-cysteine, and glycine, whereas its 3 products are ADP, phosphate, and glutathione