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Competitive association kinetics TR-FRET was monitored on simultaneous addition of four or more concentrations of unlabeled agonist plus 10 nM exendin-4-FITC to CHO-SNAP-GLP-1R cells labeled with Lumi4-Tb and pre-incubated with metabolic inhibitors

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These breakthrough findings not only confirm at the molecular level the molecular targets through which GLP-1RAs improve neurodegenerative diseases by regulating glucose-lipid metabolism pathways, but also further establish a theoretical framework for the synergistic regulation of metabolic intervention and neuroprotection (Figure 5)