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We also consider the lack of biochemical testing during the fasting period to be a significant limitation of this study

Nausea occurred in 45% of the 12 mg group, diarrhea in 29%, and vomiting in 16% [3]

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This assay is a Sandwich ELISA based, sequentially, on: 1) capture of GLP-1 Total molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti- GLP-1 polyclonal antibody, 2) wash away of unbound materials from samples, 3) binding of a biotinylated anti- GLP-1 monoclonal antibody to the captured molecules, 4) wash away of unbound materials from samples, 5) conjugation of horseradish peroxidase to the immobilized biotinylated antibodies, 6) wash away of free enzyme conjugates, and 7) quantification of immobilized antibody-enzyme conjugates by monitoring horseradish peroxidase activities in the presence of the substrate 3,3,5,5-tetramethylbenzidine