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Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine)

Apoptotic cells in lung tissues were evaluated through Terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) staining by a Roche In Situ Cell Death Detection Kit (Roche, U.S.) according to the suggested protocols

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Current markers (e.g., GPX4, 4-HNE, and serum iron) lack specificity for ferroptosis-driven RF, as they are also altered in other kidney diseases or metabolic disorders