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At defined time points (0, 30, 60, and 90 s), 40 L samples were heat inactivated and the aldehyde concentration was determined as detailed above

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However, unlike previous findings, shikonin, icariin, and other drugs induce mitochondrial dysfunction by increasing ROS levels, decreasing mitochondrial membrane potential, and elevating the release of cytochrome C and pro-apoptotic proteins, such as caspase-3 and caspase-9, to induce apoptosis in FLSs to suppress inflammatory response (265, 266)