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Hongmei Li, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

People with high insulin resistance (ie, people with obesity), young age at dysglycaemia, abnormal 1 h glucose concentrations (86 mmol/L) despite normal 2 hr glucose concentrations on 75 g oral glucose tolerance test, and low C-peptide are at higher risk for progressing to stage 3 (fast progressor, stage 2b) than those diagnosed with dysglycaemia after age 65 years (slow progressor, stage 2a)

Additionally, the designing of different sensors has used the circularly permutated fluorescent protein (cpFP), where the original N and C termini were replaced by a short peptide linker in order to alter the pKa values and orientation of chromophore from their original counterpart 11,12

doi: 10.1021/acs.chemrestox.7b00311