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Treatment of phLF and phBEC with TGF-1, TNF-, and menadione For treatment of phLF, cells were seeded at a density of approximately 20,000 cells/cm 2 , starved for 24 hours in DMEM/F12 with 0.5% FBS and 100 nM sodium selenite, followed by treatment with 2 ng/ml TGF-1 (R&D Systems, Minneapolis, MM, USA), 10 ng/ml TNF- (PeproTech, Rocky Hill, NJ, USA), and 10 M menadione (Sigma-Aldrich) in starvation medium for the indicated time points
Denicola et al., 2016)
Likewise, another dualAG named cotadutide (MEDI0382) was more effective than obeticholic acid in improving liver metabolism and ameliorating MASH and fibrosis in C57BL/6J or ob/ob mice on a high trans-fat/fructose/cholesterol diet 14 , as well as decreased liver inflammation and apoptosis in adipocytes 15
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