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The GSH- o -phthalaldehyde adducts were separated on a 4.6250 mm Luna C18 column (5 m, Phenomenex, Torrance, CA, U.S.A.) using two Waters 510 pumps, a 717 autosampler, and a 474 fluorescence detector (Milford, MA, U.S.A.), and fluorescence was detected at 420 nm with excitation at 340 nm

The result showed that the incidence rates of CVD were 1.01 (95% CI, 0.98 to 1.03) and 2.69 (95%CI, 2.55 to 2.83) per 1000 person-years in the non-MASLD and MASLD groups, respectively

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All those contributing skin samples provided informed written consent, and both the patients and volunteers expressed agreement for the publication of their photographs