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Mioduchoski, FNP-BC, is a board-certified Family Nurse Practitioner providing physician-supervised medical weight loss, GLP-1 support, peptide therapy, and wellness-focused telehealth care through GoalBMI Wellness

According to the manufacturers recommendation, mice were initially weighed and transferred individually into the 8 metabolic cages with free access to food and water

Killed mice were then perfused transcardially with 50 ml of cold phosphate buffered saline (PBS), the meninges were removed, and the brain was blotted dry