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To further investigate whether high glutamine-induced melanoma growth inhibition is mediated by histone hypomethylation, we cultured two parental patient-derived melanoma cells, M229 and M249, in tumour-like conditions, under low glutamine (0.5 mM) and 1% oxygen

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Pooled libraries were sequenced on a NextSeq 2000 (Illumina) using 100-bp single-end sequencing

We adjust laser parameters based on skin tone, hair color, and any skin sensitivities before every session