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qRTPCR was performed via SYBR Premix Ex Taq II (Takara) on a Chromo 4 continuous fluorescence detector with a PTC-200 DNA Engine Cycler (Bio-Rad Laboratories, Hercules, CA)

La 21-OHD no clsica (NC21OHD) se refiere a la condicin en que las deficiencias parciales de 21-hidroxilacin producen menos hiperandrogenemia extrema y sntomas ms leves