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Q4: Is GHK-Cu better topical or injectable

Cells were treated with various concentrations of URB597 or 4-(3-chloro-2-pyridinyl)-N-[4-(1,1-dimethylethyl)phenyl]-1-piperazinecarboxamide (BCTC) (0.02, 0.5, 2 mol/L, Cayman Chemical Company, Ann Arbor, MI, USA), SR144528 (0.02, 0.5, 2 mol/L, EMD Millipore, Etobicoke, ON, Canada), indomethacin (2, 20, 50 mol/L, Sigma-Aldrich, Oakville, ON, Canada), or vehicle solution (0.1% dimethyl sulfoxide, DMSO) and incubated for 15 min at 37 C in 5% CO 2

Yue X, Kiesewetter DO, Guo J, Sun Z, Zhang X, Zhu L, Niu G, Ma Y, Lang L, Chen X

Serotonin, tryptophan metabolism and the brain-gut-microbiome axis