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Functionally, this predicted cleavage site is only 5 residues from the C terminal of TXNRD1, and would result in removal of the C-terminal redox center of TXNRD1 (AGCUG), making the enzyme incapable of regenerating reduced thioredoxin

N Engl J Med 369(4):362372

Shaking creates foam air bubbles trapped against peptide molecules which can denature the peptide at the air-liquid interface

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