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This variant does not catalyze the typical thioltransferase and reductase reactions that are a feature of the Omega class GSTs C47A/C101A mutant shows 41% decrease in the specific activity towards 1-chloro-2,4-dinitrobenzene compared to the His-tagged wild type enzyme C78A - mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity C87A/C115A/C174A/M212C - site-directed mutagensis of isozyme GST M2-2, mutation of the catalytic site residue M212 and of all cysteines, the mutant shows altered substrate specificity compared to the wild-type isozyme