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Following an acute administration of EtOH (5 g/kg, i.p.), KO animals exhibited 30% and 50% less EtOH and AA accumulation in circulation, respectively, suggesting an increased capacity for liver EtOH and AA metabolism in KO mice

Mant and colleagues describe HPLC as a core approach for peptide analysis and purification, including several chromatographic modes used in peptide work [9]

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Next, plate was washed 3 times and each well was incubated with 100 L of TMB in the dark for 15 min at room temperature followed by 100-L of acidic stop solution