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For each transfection, 5 l of Lipofectamine 2000 (11668019, Invitrogen) was added to 100 l of OptiMEM (Gibco), vortexed briefly and incubated for 5 minutes at room temperature

[144] Hanks appeared in Wes Anderson's Asteroid City (2023), starring alongside Jason Schwartzman, Scarlett Johansson, Adrien Brody, Jeffrey Wright and Bryan Cranston

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However, it is important to consider that patterns of body weight and adipose tissue distribution vary significantly across different racial groups [22]