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2.4 Sample preparation For LCQTOF-MS/MS analysis, 1 mg of powder or homogenized tablet was dissolved or extracted using 5 mL of a 1:1:1 (v/v/v) mixture of water/methanol/acetonitrile, assisted by ultrasonication for 10 min, and afterwards filtered by a Whatman 0.2 m pore size polytetrafluoroethylene (PTFE) filter medium (GE Healthcare, Chicago, IL, United States)

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The adverse effects of aspirin such as tinnitus, Reyes syndrome and gastrointestinal bleeding are caused due to conversion of aspirin into its active metabolite salicylic acid after oral intake

The currently recommended pharmacological treatments for DPN mainly include antidepressants due to their analgesic effects, while a strategy for the pathogenesis and progression of DPN is lacking