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Multifaced roles of the v3 integrin in ehlers-danlos and arterial tortuosity syndromes' dermal fibroblasts

PASMC Proliferation Assay Primary PASMCs were isolated and maintained in culture after the protocol outlined in our previous study

the collected blood was put in a gel and clot activator tube and left in the stand for 30 min, then centrifuged for 15 min at 5000 rpm at room temperature

Accumulation of ethanol-associated reactive molecules, e.g., reactive oxygen (ROS) and nitrogen species (RNS), and electrophilic products, e.g., acetaldehyde and lipid peroxidation-derived products, can be harmful to biological systems due to their propensity to inactivate enzymes, leading to loss of protein function, DNA damage and cell death 4