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Transwell migration assay L929 cells (1 10 5 cells per well) were seeded into the upper chamber (8 m pore size)

Because hydrophobic bile acids are harmful to hepatocytes and cholangiocytes, obstruction of bile flow causes retention of bile acids in the liver, resulting in liver damage (Nguyen et al., 2014

This compound has been studied in controlled research models for its interaction with melanocortin receptors, particularly within central nervous system signaling pathways