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For the behavioral experiment of SPF-Sham and SPF-FUS, mice were tested OFT at 9 weeks of age, CCK-8 injected food intake at 10 weeks of age, saline-injected 11 weeks of age, and motor function test (wire hanging test, ledge test, and hindlimb clasping test, and rotarod test) at 12 weeks of age

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The standard starting dose protocol begins at 2.5 mg for a reason

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