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RNA was isolated from liver and cell samples using TRIzol reagent (Life Technologies, Thermo Fisher Scientific, Waltham, MA, United States) and converted to cDNA using a reverse transcription kit from Thermo Fisher Scientific

5 Conclusion Given the limited epidemiological knowledge about malignant melanotic nerve sheath tumors (MMNSTs), our study contributes to the literature by documenting a cervical intra- and extraspinal MMNST without previously described driver mutations or copy number changes