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Third, the absence of vascularization and perfusion in static PDCO cultures restricts nutrient diffusion and compromises physiological relevance

Convert vial amount to micrograms (if needed): mg 1,000 = mcg Concentration: total amount (mcg) total liquid (mL) = mcg/mL Draw volume: target amount (mcg) concentration (mcg/mL) = mL U100 units: mL 100 = syringe units Worked example (math only) If a vial is labelled 5 mg (5,000 mcg) and you add 2 mL, the concentration is 2,500 mcg/mL

Sterile water has no preservative Plain sterile water contains no antimicrobial agent, so once a vial is punctured, any unused solution should be discarded rather than stored for later draws