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These changes often occur due to rapid fat and in some cases, muscle loss, which can leave skin looking more loose and aged, particularly on the face and buttocks

After the cells reached ~ 80% confluence under normal culture conditions, the medium was changed to glucose-free DMEM (Gibco) and the dish was placed in an anaerobic chamber (5% CO2, 95% N2) to induce OGD-related cell injury

Stop after six months and the weight is likely to return unless something else changes