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Serial diluted peptideantibody conjugates were incubated with 40,000 cells in assay buffer (0.1% BSA, 500 M 3-isobutyl-1-methylxanthine in F12 media) for 15 min at 37 C

Tirzepatide dosing protocol Tirzepatide follows a well-established escalation schedule refined through multiple clinical trials and years of real-world use: Weeks 1-4: 2.5 mg once weekly (starting dose) Weeks 5-8: 5 mg once weekly Weeks 9-12: 7.5 mg once weekly (optional intermediate step) Weeks 13-16: 10 mg once weekly Weeks 17-20: 12.5 mg once weekly (optional intermediate step) Week 21+: 15 mg once weekly (maximum dose) The starting dose of 2.5 mg is intentionally subtherapeutic