The X49 for myself, and the IceWave to carry around with me and give them to people in pain (which would make for a decent single-blind test)
Tolerance to apoptotic cells is regulated by indoleamine 2,3-dioxygenase
At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore
Methods Reagents and tools table Cell lines and cell culture HEK293T, HEK293T RSMC heterozygous knockout (RSMC +/ ), HEK293T Flag-Sororin, HeLa, dCad9- GFP 14 -integrated HeLa, HeLa RSMC-3Flag, HeLa PARP1 WT and KO (provided by X Xu) cells were grown in DMEM (Gibco,11995500BT) supplemented with 10% FBS (ABW, AB-FBS0500) and antibiotics