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Reactive oxygen species (ROS) or free radicals cause damage to key cellular components like DNA, RNA, lipids, proteins, and structural membranes

Enzyme Activities For enzyme activities, cells and mouse tissues (duodenum, liver, spleen) were homogenized with a TissueLyser II (Qiagen, Hilden, Germany) in Tris buffer (100 mM Tris (Carl Roth), 300 mM KCl (Applichem, Darmstadt, Germany), pH 7.6 with 0.1% ( v/v ) Triton X-100 (Serva, Heidelberg, Germany), and 0.1% ( v/v ) protease inhibitor (Merck/Millipore, Burlington, MA, USA)) two times for 60 s at 30 Hz

The exhaustion of sleepless nights, hormonal shifts, emotional eating, and lack of time often make traditional exercise routines feel impossible

The authors conclude that accelerated gastric emptying following gastric sleeve surgery increases GLP-1 secretion by distal intestinal L cells most likely by increasing undigested nutrient transit across the small intestine