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For T4 treatment, control and APP2 cerebral organoids received 0.5 g/mL of T4 in their Maturation Medium

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It mimics two natural hormones in the bodyGLP-1 and GIPthat play key roles in regulating appetite and blood sugar levels

In further studies, only the formation of [14C]carbon dioxide from [14C-1]glucose was measured, with the addition of: sodium ascorbate (which undergoes a non-enzymic reaction in air to produce hydrogen peroxide) acetylphenylhydrazine (which is known to precipitate haemolysis in sensitive subjects, and depletes glutathione) methylene blue (which oxidises NADPH) All incubations were repeated in the presence and absence of N -ethylmaleimide, which undergoes a non-enzymic reaction with the -SH group of reduced glutathione, and thus depletes the cell of glutathione