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doi: 10.1517/17425247.3.5.685 196

The GSH/GSSG redox potential has become a fashionable electrochemical parameter believed to be a major driving force of redox reactions regulating biological events (Schafer and Buettner, The impact of glutathione on biological redox events Whoever tried to directly determine redox potentials of proteins electrochemically will not forget the boring minutes or hours of waiting until the needle of the potentiometer had come to rest

Transfection, BSO treatment, and exposure of AQ-QI and DEAQ-QI were identical as described in section Transient Transfection of Human GSTP1 Gene and Cell Viability Assay. After exposure the medium was gently removed and the later steps were conducted according to manufacturers manual