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An aliquot of 20 l of the third-round virus mixture was used to infect 500 ml (SFM900-II medium with 5% FBS, Thermo Fisher) of Sf9 cells (1.5 10 6 /ml) for protein expression in an orbital shaker (Shanghai Zhichu Instrument) at 27 C and 200 rpm

Combination of topical and oral glutathione as a skin-whitening agent: a double-blind randomized controlled clinical trial

Nuclei were pelleted by centrifugation (650 g, 5 min), supernatant was discarded and the pellet was resuspended in 100 l NUN1 buffer containing 20 mM Tris-HCL (pH = 7.5), 75 mM NaCl, 0.5 mM EGTA, 50 % glycerol, 1x protease and 1x phosphatase inhibitors

Adding healthy mitochondria eradicates depression symptoms: Transplanting healthy mitochondria into the brains of mice cures depressive symptoms