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The sections were then blocked in blocking solution (PBS, 0.1% Tween-20, 1% donkey serum, 5% FCS) for 1 h, followed by incubation with the primary antibodies overnight at 4 C

Preliminary cardiovascular outcome data on tirzepatide in type 2 diabetes The above-mentioned preliminary cardiovascular safety data from the SURPASS-4 study demonstrated no excess cardiovascular risk associated with tirzepatide therapy (54, 73)