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In order to eliminate the precipitated protein, the supernatant was deproteinized using a 10% trichloroacetic acid (Sigma-Aldrich) solution and centrifuged at 10,000 g for 10 min at 4 C

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Gla residues are usually involved in binding calcium, and are essential for the biological activity of all known Gla proteins

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Kapoor K, Alfaddagh A, Al Rifai M, Bhatt DL, Budoff MJ, Nasir K,

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