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388 To confirm the role of GLP-1 in enhancing physical endurance and the possible mechanisms involved, an in vivo AAV-mediated GLP-1 overexpression model and an in vitro siRNA-mediated AMPK knockdown model were generated

A microplate reader (Synergy Mx, Biotek Instruments Inc., Winooski, VT, USA) were used for determination of all activities and scaled down for semi-high throughput to obtain linear time and protein concentration dependence

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