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2.6 Immunofluorescence Staining Frozen pancreatic sections (10 m) were prepared using a Leica cryostat

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Several examples have also now emerged suggesting the GH/IGF-1 signaling pathway is relevant to human aging 12 , including the discovery of functional mutations in the IGF-1R gene in individuals with exceptional longevity, resulting in relative IGF-1 resistance 13,14 , and in subjects lacking functional GH receptors (Laron dwarfs) 15

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