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This hypothesis has been recently supported and expanded by the SELECT trial, including patients with a BMI 27 kg/m 2 and a history of CVD, but without type 2 diabetes, in which the rate of HF complications was reduced by semaglutide treatment and this protective effect occurred early after initiation of the trial, i.e., before substantial weight loss could be achieved [37]

Ten ul of CCK-8 solution in 100 ul culture medium was added to the wells before a 2 h incubation at 37 C

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