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Handling and Aseptic Technique Proper handling is essential to maintain the integrity of BAC Water in research use: Withdrawals should be performed with sterile syringes or equipment The stopper and contact surfaces should be disinfected prior to each use Containers should be stored in accordance with laboratory guidelines and discarded if contamination is suspected While the solution is bacteriostatic, not bactericidal, adherence to aseptic technique is still required to minimize experimental variability and contamination risk

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In addition, the decrease in bone tissue hardness may lead to cartilage deformation and cartilage pathology, which are associated with osteoarthritis (Stewart and Kawcak, 2018)