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Do Not Shake : Gently swirl the vial to mix the solution if needed, but avoid vigorous shaking, which can cause bubbles and potentially degrade the medication

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Collectively, our results from evolving a redesigned protease side-by-side with the corresponding WT protease to cleave three new substrates demonstrate that the enhanced stability of the D3 starting point protease enabled, across all evolutions, higher evolved function and the productive use of mutations that function poorly with the WT protease