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doi: 10.1016/j.jsbmb.2018.01.014 92 SargisRMJohnsonDNChoudhuryRABradyMJ

The different stratified skin layers were suspended in methanol and the copper peptides were extracted by ultrasonication

Insulin concentrations of both supernatants and cellular contents were determined using Insulin (human) AlphaLISA Detection Kit (PerkinElmer)

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