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Intracellular ROS Measurement The intracellular ROS production in H9c2 cells was assessed using a fluorescent dye, 2,7-dichlorodihydrofluorescein diacetate (H 2 DCF-DA) (Sigma-Aldrich, USA) as described previously ( 5 cells/well) in a 12-well plate overnight before treatment with various agents in serum-free medium condition

In a large-animal pharmacokinetic study, the HeroPatch array delivered a GLP-1 dose equivalent to a 3.6 mg subcutaneous injection of semaglutide, the active ingredient in obesity and Type 2 diabetes drugs

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