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Previous reports have demonstrated the occurrence of the conjugation of GSH to azo esters 35 or diamides 36
Additionally, the development of site-specific conjugation technologies for linkers has made it possible for each antibody molecule to carry a uniform number of payloads (resulting in a homogeneous drug-to-antibody ratio, DAR), enabling positional control, maximally reducing product heterogeneity, and preserving key epitope-binding regions [94]
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