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Primer sequences: TGF-: F: 5'-TGCTAATGGTGGACCGCAA-3', R: 5'-CACTGCTTCCCGAATGTCTGA-3' TNF-: F: 5'-CCAGGAGAAAGTCAGCCTCCT-3', R: 5'-TCATACCAGGGCTTGAGCTCA-3' IL-6: F: 5'-GCCCTTCAGGAACAGCTATGA-3', R: 5'-TGTCAACAACATCAGTCCCAAGA-3' GAPDH: F: 5'-CATGTTCGTCATGGGGTGAACCA-3', R: 5'-AGTGATGGCATGGACTGTGGTCAT-3' Statistical Analysis Data were analyzed using SPSS (version 26) and are expressed as mean standard deviation (SD)