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Determination of serum transaminases Serum levels of hepatic function markers alanine aminotransferase (ALT), aspartate aminotransferase (AST), and creatinine were determined using commercial high-quality optimized Elabscience ELISA test kits (MD, USA) in a microplate reader, according to the manufacturers instructions (Zhao et al

This approach requires two separate vials and more planning, but it optimizes both precision and convenience across the protocol

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