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At the N-terminus, the Tyr1 of Tirzepatide makes Van der Waals contact with the receptor residues, the Glu3 forms an ionic bond with Arg183 and a network of hydrogen bonds with Tyr145 and Ser381 of the receptor

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bauammnii ZigA reveal that all three Cys residues contribute to a S 3 (O/N) Zn II coordination complex in the G-nucleotide-free form, with the identity of the fourth ligand as yet unknown

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