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One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)

When the destructive elements are fully activated in microglia, they will show strong inflammatory and excitatory toxic reactions, exceeding the response level of physiological activation of microglia (Norden et al., 2015)

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