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It was also indicated that the fluorescence properties of Trp residues in the proteins can be described based on the existence of three discrete spectral classes, a buried in nonpolar regions of the protein ( max = 330332 nm, spectral band width = 4849 nm, quantum yield q = 0.11, and lifetime = 2.1 ns), a completely exposed to water ( max = 350353 nm, = 5961 nm, q = 0.2, = 5.4 ns)

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